Bacteriostatic Water for Peptide Reconstitution

Reconstitution Solvent - Multi-Dose Safe - USP Grade

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0.9% Benzyl Alcohol Sterile Diluent | 30mL Multi-Dose Vial

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The essential companion to every peptide protocol - USP-grade sterile water preserved with 0.9% benzyl alcohol for safe, stable multi-dose reconstitution without the risk of bacterial contamination

Bacteriostatic Water (BAC Water) is sterile water for injection preserved with 0.9% benzyl alcohol - a widely accepted antimicrobial agent that inhibits bacterial growth and makes the solution safe for use across multiple draws from the same vial. It is the industry-standard diluent for reconstituting lyophilized (freeze-dried) peptides including BPC-157, TB-500, Semaglutide, GHK-Cu, Thymosin Alpha-1, and other research compounds. Unlike sterile water for injection, which must be used immediately after opening, BAC Water remains bacteriostatic for up to 28 days after the first use, making it the preferred choice for multi-dose peptide vials.

USP-grade sterile water with 0.9% benzyl alcohol - inhibits microbial growth for safe multi-dose use
30mL multi-dose vials - sufficient for reconstituting multiple peptide vials per bottle
Compatible with all common lyophilized research peptides - BPC-157, TB-500, Semaglutide, GHK-Cu, and more

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Why BAC Water Is the Gold Standard for Peptide Reconstitution

When a peptide arrives as a lyophilized (freeze-dried) powder, it must be dissolved into a sterile liquid before it can be injected. The choice of diluent has significant implications for both safety and stability. Bacteriostatic Water has become the universal standard for peptide reconstitution for one simple reason: the 0.9% benzyl alcohol it contains acts as a preservative, inhibiting bacterial growth across repeated entries into the vial - unlike plain sterile water, which is intended for single-use only.

Each 30mL vial contains enough BAC Water to reconstitute multiple peptide vials, making it an efficient and economical part of any protocol. The benzyl alcohol concentration has been validated across decades of pharmaceutical and clinical use as safe, non-toxic at standard reconstitution volumes, and effective at maintaining sterility for up to 28 days post-puncture.

Key Properties

010.9% benzyl alcohol preservative - inhibits all common bacterial contaminants across multiple draws
02USP-grade sterile manufacturing - produced to pharmaceutical-grade standards, pyrogen-free
03Stable for 28 days after first use - far superior shelf life vs. sterile water for injection
04Compatible with all common peptide sequences - no reactivity with lyophilized research peptides
0530mL per vial - economical; one BAC Water vial typically covers an entire peptide protocol cycle

Product Specifications

Product Name	Bacteriostatic Water for Injection
Preservative	0.9% Benzyl Alcohol (USP)
Volume	30mL per vial
Grade	USP-grade, pyrogen-free, endotoxin-tested
Sterility	Aseptically manufactured; sterility verified
In-use Stability	28 days from first puncture (refrigerated)
Storage	Room temperature prior to opening; refrigerate after first use
Price	From $12.00 per vial

Compatibility Reference

BPC-157 - 2mL BAC Water per 5mg vial (standard 500mcg/mL solution)

TB-500 (Thymosin Beta-4) - 2mL BAC Water per 5mg vial

Semaglutide / Tirzepatide - 1-2mL BAC Water per vial per provider protocol

GHK-Cu - 1mL BAC Water per 50mg vial (standard 50mg/mL solution)

Thymosin Alpha-1 - 1mL BAC Water per 5mg vial

Reconstitution volumes vary by protocol. Always follow the guidance of your supervising healthcare provider.

Step 1: Gather Supplies

You will need: the lyophilized peptide vial, a vial of Bacteriostatic Water, two sterile syringes (one for drawing BAC Water, one for dosing), and alcohol prep swabs. Wash hands thoroughly and work on a clean, flat surface. All equipment should be sterile and single-use.

Step 2: Draw the BAC Water

Wipe the rubber stopper of the BAC Water vial with an alcohol swab and allow it to dry. Using a sterile syringe, draw the required volume of Bacteriostatic Water as specified by your healthcare provider (typically 1-2mL per peptide vial depending on the desired concentration). Pull the plunger slowly to avoid introducing air bubbles.

Step 3: Inject into the Peptide Vial

Wipe the rubber stopper of the peptide vial with a fresh alcohol swab. Slowly inject the Bacteriostatic Water down the side of the vial - do not inject directly onto the lyophilized powder cake, as this can degrade the peptide. Allow the water to trickle down the glass and dissolve the powder naturally. Do not shake; gently swirl or roll the vial between your palms until the solution is fully clear.

Step 4: Store and Use Within 28 Days

Once reconstituted, store the peptide vial in the refrigerator (2-8C) and protect it from light. Use the reconstituted solution within 28 days. Label the vial with the date of reconstitution. Each time you draw a dose, wipe the stopper with a fresh alcohol swab. The BAC Water vial itself should also be refrigerated after opening and used within 28 days.

Who Needs Bacteriostatic Water?

Bacteriostatic Water is required by anyone reconstituting a lyophilized peptide or injectable compound for subcutaneous or intramuscular administration. It is not optional - using tap water, distilled water, or saline is inappropriate and potentially dangerous. BAC Water is specifically engineered to maintain sterility across the multiple draw events that characterize peptide protocols spanning weeks to months.

Ideal For

+Healthcare providers and clinic staff managing lyophilized peptide and compounded injectable protocols
+Anyone running a BPC-157, TB-500, Semaglutide, GHK-Cu, or Thymosin Alpha-1 protocol requiring reconstitution
+Longevity clinics and wellness practices maintaining multi-dose peptide vial inventories for patient protocols
+Practitioners seeking a reliable, cost-effective, pharmaceutical-grade diluent for their reconstitution workflow

Important Notes

-Not for use in neonates or newborns - benzyl alcohol has been associated with a fatal toxic syndrome in premature neonates; always use benzyl alcohol-free sterile water for pediatric injectable preparations
-Check peptide-specific compatibility - while BAC Water is appropriate for most peptides, some specialized formulations may specify alternative diluents
-Do not use if vial integrity is compromised - inspect for cracks, particulate matter, or cloudiness before use

Reconstitution and administration should be performed under the guidance of a licensed healthcare provider. See Important Safety Information below.

The critical difference is the preservative. Bacteriostatic Water contains 0.9% benzyl alcohol, which inhibits bacterial growth and allows the vial to be used for multiple draws over up to 28 days. Sterile Water for Injection (SWFI) contains no preservative and is intended for single-use only - once the vial is punctured, it must be used immediately and discarded, as there is nothing to prevent bacterial contamination of subsequent draws. For peptide protocols that require repeated dosing from the same vial, BAC Water is always the correct choice.

The volume depends on the desired final concentration and the peptide vial size. As a general reference: BPC-157 5mg vials are commonly reconstituted with 2mL of BAC Water (giving a 2,500mcg/mL solution), TB-500 5mg vials with 2mL, and GHK-Cu 50mg vials with 1mL. Semaglutide and Tirzepatide concentrations vary significantly by protocol. Always follow your supervising healthcare provider's specific instructions for your protocol, as concentrations directly determine dosing calculations.

Once opened (first vial puncture), Bacteriostatic Water remains sterile and suitable for use for up to 28 days when stored in the refrigerator (2-8C). Always label the vial with the date of first use and discard after 28 days regardless of how much remains. Similarly, once a peptide has been reconstituted with BAC Water, it should also be used within 28 days and stored refrigerated.

Yes. Bacteriostatic Water with 0.9% benzyl alcohol is a well-established pharmaceutical-grade diluent with decades of clinical use. Benzyl alcohol at 0.9% concentration is well below the threshold for toxicity in adults and has been validated as safe across an enormous volume of clinical experience with injectable medications. The primary exception is neonates, where benzyl alcohol is contraindicated. Our BAC Water is manufactured to USP specifications, pyrogen-free and endotoxin-tested.

At the most common reconstitution volume of 2mL per peptide vial, a single 30mL BAC Water vial can reconstitute up to 15 peptide vials. At 1mL per peptide vial, up to 30 vials. In practice, most users running a single peptide protocol find that one 30mL BAC Water vial comfortably covers an entire treatment cycle.